aml12 normal mouse liver cells  (ATCC)

Journal: Discover Oncology

Article Title: Isosilybin B: a potential novel therapeutic agent with hepatoprotective, anticancer and antifibrotic properties

doi: 10.1007/s12672-025-03380-8

Figure Lengend Snippet: Effect of IB/SB/SM on cytotoxicity and cell cycle arrest of liver cells. A : Chemical structure of IB and SB (silybin A and silybin B in ratio 1:1). Cytotoxicity in Hepa1-6 ( B ), HepG2 ( C ) or AML12 ( D ) liver cells after 24 h treatment with IB/SB/SM. Changes in the cell cycle in Hepa1-6 ( E ), HepG2 ( F ), or AML12 ( G ) liver cells after 24 h treatment with 31.3 µg/mL of IB/SB/SM. H : Cell distribution of diploid cells in the phases of the cell cycle shown for IB (31.3 µg/mL). The data are given as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Mouse normal liver hepatocyte cell line AML12 (RRID: CVCL_0140, ATCC, USA) was cultivated in DMEM/F12 medium supplemented with 10% FBS, 1% penicillin-streptomycin, 1X Insulin-Transferrin-Selenium and 40 ng/mL dexamethasone.

Techniques:

Journal: Discover Oncology

Article Title: Isosilybin B: a potential novel therapeutic agent with hepatoprotective, anticancer and antifibrotic properties

doi: 10.1007/s12672-025-03380-8

Figure Lengend Snippet: Effect of IB/SB/SM on pro-fibrotic gene expression, antioxidant activity, and ALT concentration in AML12 cells. (A-C) Expression of pro-fibrotic genes fibronectin ( Fn1 ), smooth muscle actin ( Acta2 ) and collagen I ( Col1a1 ) after 24 h treatment with TGF-β1 (10 ng/mL) and IB/SB/SM (7.8–31.3 µg/mL) in AML12 cells. (D) Western blot analysis of fibronectin expression in AML12 cells stimulated for 24 h with TGF-β1 (10 ng/mL) and IB/SB/SM at concentration 31.3 µg/mL. Representative western blot bands are shown below the graphs. (E) DPPH antioxidant activity assay. The ability of IB/SB/SM to scavenge DPPH radicals was evaluated at various concentrations (0–500 µg/mL). (F) Determination of alanine aminotransferase (ALT) concentration in the supernatants of AML 12 cells stimulated with TGF-β1 (10 ng/mL) in the presence of the tested compounds (IB, SB, SM) at different concentrations. All results are expressed as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0,001 (for western blot: vs. TGF-β1 control)

Article Snippet: Mouse normal liver hepatocyte cell line AML12 (RRID: CVCL_0140, ATCC, USA) was cultivated in DMEM/F12 medium supplemented with 10% FBS, 1% penicillin-streptomycin, 1X Insulin-Transferrin-Selenium and 40 ng/mL dexamethasone.

Techniques: Gene Expression, Antioxidant Activity Assay, Concentration Assay, Expressing, Western Blot, Control